Recent developments in the isolation and quantification of extracellular vesicles (EVs) from breast cell lines
BMC Seminar Thursday 18 February, 12:00
Speaker: Berglind Eva Benediktsdóttir, Associate Professor, Faculty of Pharmaceutical Sciences, University of Iceland
Title: Recent developments in the isolation and quantification of extracellular vesicles (EVs) from breast cell lines
Abstract: EVs are nanosized vesicles released from human cells. They participate in cell-to-cell communications by transporting proteins, mRNA and miRNA leading to functional change in the recipient cell. As such they participate in various physiological functions such as red blood cell maturation, inflammatory response and angiogenesis but they are also implicated in diseases such as neurodegenerative diseases, HIV and cancer. The intrinsic ability of EVs to deliver proteins and miRNAs has sparked interest of their utility as a nanosized drug carriers. To be able to use EVs in marketed formulations, the isolation method needs to be scalable, have good reproducibility, operator independent and yield pure, biologically active EVs. The D492 and the MDA_MB_231 cell lines originate both from the breast epithelium, yet have very different origin (non-cancerous vs. cancerous). The former has potential to produce EVs for drug delivery purposes but the latter useful for comparison as it is of cancerous nature. In this BMC seminar I will take you through the world of EVs as they relate to drug delivery and isolation strategies, then zoom in on the current status of EV isolation from the above-mentioned cell lines using SEC and centrifugal filtration, and how Jess (capillary western blotting) has helped in confirming the isolation of EVs.
Zoom link: https://eu01web.zoom.us/j/65809434042
Berglind Eva Benediktsdóttir, Associate Professor, Faculty of Pharmaceutical Sciences, University of Iceland
