Metabolic profiling on formalin-fixed paraffin-embedded breast cancer tissue using desorption electrospray ionization mass spectrometry imaging
BMC Seminar Thursday 18 March, 12:00
Speaker: Ólöf Gerður Ísberg, PhD student in Pharmaceutical Sciences at University of Iceland and at Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, UK. Supervised by Prof. Margrét Þorsteinsdóttir, Sigríður Klara Böðvarsdóttir and Prof. Zoltan Takats.
Title: Metabolic profiling on formalin-fixed paraffin-embedded breast cancer tissue using desorption electrospray ionization mass spectrometry imaging
Abstract: A broad range of diseases are identified based on histology. Today, most pathological tissue samples are stored as formalin-fixed paraffin embedded (FFPE) tissue blocks, which is the gold standard for histopathological analysis. Mass spectrometry imaging (MSI) is a powerful tool that enables us to investigate the regional distribution of a variety of molecules in biological samples. Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is particularly suited to investigate the spatial distribution of metabolites. Metabolite measurements are growing in importance as altered metabolism is a hallmark of many diseases, such as cancer. Currently, fresh frozen (FF) samples are preferred over FFPE samples in tissue based MSI studies. This is primarily due to the concern that metabolites will be lost during fixation and chemical processing. My results indicate that we are able to detect metabolites in FFPE samples.