BMC Seminar Thursday 28th of February at 12:00 in room 201 Læknagarður
Speaker: Kirstine Nolling Jensen, PhD student supervised by Ingibjörg Harðardóttir and Jóna Freysdóttir, Department of Immunology, University Hospital and Faculty of Medicine, University of Iceland.
Title: Dietary fish oil enhances peritoneal NK cell maturation and may modulate Wnt signaling in murine antigen-induced peritonitis
Abstract: We have previously demonstrated that dietary fish oil enhances resolution of antigen-induced inflammation in mice. The fish oil diet also led to an early peak in peritoneal NK cell numbers, and in a follow-up study we showed that NK cell depletion resulted in incomplete resolution of inflammation. The objective of the present study was to determine the effects of dietary fish oil on NK cell maturation and on expression of inflammation related molecules. Mice were fed either control (C) or fish oil (FO) diet, immunized twice with methylated BSA (mBSA) and challenged by injecting mBSA into their peritoneum. Peritoneal cells and fluid were harvested at the peak of inflammation (6 h). NK cells were sorted for RNA sequencing, expression of surface molecules determined by flow cytometry and concentrations of selected inflammation related molecules determined by Luminex. The FO fed mice had a higher number of terminally differentiated NK cells than mice fed the C diet, but fewer intermediately differentiated NK cells. RNA sequencing indicated that three molecules related to Wnt signaling were differentially expressed in mice fed the C and the FO diet. The FO fed mice had a higher peritoneal concentration of DKK-1, a Wnt signaling inhibitor. Furthermore, lower concentrations of IL-6, IL-6Rα and CCL20, all products of Wnt signaling, were detected in the FO group. These data demonstrate that dietary fish oil modulates NK cell maturation and molecules involved in Wnt signaling in NK cells suggesting a role for NK cells and Wnt signaling in resolution of inflammation.