Friday, May 5, 2017 - 12:00
Specific location: 
Room 343

Speaker: Erika MoreraPhD student. Department of Medical Anatomy, and Department of Laboratory Hematology, University of Iceland.

Title: The endothelial niche in breast morphogenesis and cancer

Subtitle: Comparison of two isogenic non-tumorigenic and tumorigenic breast epithelial- derived cell lines with mesenchymal phenotype.

Abstract: D492 is a breast epithelial cell line with stem cell properties that can generate both luminal and myoepithelial cells and in 3D culture it forms branching terminal ductal lobular units TDLU-like structures. D492M was generated by co-culture of D492 and breast endothelial cells (BRENCs) that resulted in subpopulation of cells undergoing epithelial to mesenchymal transition giving rise to D492M. D492HER2 was generated by overexpression of HER2 in D492 and this also resulted in a cell line showing EMT phenotype. D492M is non-tumorigenic while D492HER2 is highly tumorigenic. The objective of this project is to compare these two cell lines because even though both cell lines show EMT phenotype, they differ in tumorigenicity.


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