BMC Seminar: Thursday, 19th January at 12:25, Room 343, Læknagarður
Speaker: Amaranta Armesto, PhD student. Department of Medicine, University of Iceland.
Title: A role for MUC5B promoter polymorphism in idiopathic pulmonary fibrosis
Abstract: Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with high morbidity and mortality. A key feature of this disease is a pathological higher expression of the Mucin 5B (MUC5B). A common MUC5B promoter polymorphism (rs35705950) has been associated frequently in vivo with increased risk of IPF and higher expression of MUC5B both in normal and diseased lung tissue. However, little is known about the mechanism underlying this different expression and its involvement on IPF. In our research we are trying to clarify this relation analyzing the promoter area of MUC5B with both, wild type and T variation. Using our bronchial cell lines, Bci-NS1.1 and VA-10, in monolayer and air-liquid interface we have established a strong relation between the presence of the T polymorphism and an increased expression of MUC5B in vitro. By informatics approach we are also working to clarify the transcription factors involved on the signaling pathway. Several transcription factors have been analyzed in correlation with its affinity to the promoter due to the presence or absence of the wild type genotype. To fully understand the role of different transcription factor will be crucial to have a completely developed bronchial epithelium. Therefore in our research we are improving new methods as bronchosferes with stable cell lines, a faster and economic way to get a differentiated epithelium that will guarantee the natural environment for our research.