BMC Seminar Thursday 24th of January at 12:00 in room 201 Læknagarður - note a different room than usual
Speaker: Anna Karen Sigurðardóttir, PhD student of Prof. Þórarinn Guðjónsson, Faculty of Medicine, University of Iceland
Title: Expression, regulation and role of PXDN, a collagen IV crosslinker, in the mammary gland
Abstract: Branching morphogenesis is an integral process in the development of the breast gland, which is instigated through the activity of stem cells in the terminal duct lobular unit (TDLU). An important mechanism during branching is epithelial to mesenchymal transition (EMT) a process that is often hijacked in fibrosis and cancer formation. D492 is a breast epithelial progenitor cell line that generates TDLU-like structures in 3D culture and in co-culture with endothelial cells undergoes EMT that has given rise to the D492M cell line. We have shown that D492 and D492M differ greatly in terms of gene and microRNA expression.
One of the most downregulated miRNAs in D492M compared to D492 is miR-203a that has previously been shown to target EMT transcription factors such as SNAI1 and SNAI2. When we overexpressed miR-203a in D492M we observed that the most downregulated gene was peroxidasin (PXDN), which we subsequently confirmed as a novel target for miR-203a.
Our preliminary data shows that PXDN is expressed in epithelial cells and fibroblasts in the breast, as well as in fibrotic foci within the stroma.
We aim to investigate the role of PXDN in mammary gland development and disease and the molecular mechanisms behind the regulation of PXDN.