BMC Seminar Friday, 26th August at 12:00 Room 343 Læknagarður
Speaker: Sigurður R Guðmundsson, PhD student in the Eskelinen group at the University of Helsinki working on visualizing the first steps of autophagy using CLEM. Before he recived B.Sc and M.Sc from the University of Iceland, and worked for Roche Nimblegen, Iceland.
Title: Correlative light electron microscopy (CLEM) - Finding a needle in a haystack
Abstract: CLEM means observation and analysis of one and the same event or structure by both a light and electron microscopy techniques.
CLEM is a powerful tool to visualize the ultrastructure of dynamic and rare events. The strength of CLEM is the combination of the functional imaging offered by fluorescent microscopy with the structural data obtained with electron microscopy.
In this talk Sigurður will present a typical workflow of room temperature CLEM of resin-embedded cells as well as introduce different approaches used for CLEM. These include pre embedding CLEM, cryo CLEM, freeze substitution and volumetric CLEM as well as immunolabeling and the use of markers such as quantum dots, MiniSOG and APEX.